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Part:BBa_K2835002:Design

Designed by: Hanna Norbäck   Group: iGEM18_Stockholm   (2018-08-21)


Highly engineered mutant RFP (compatible with all iGEM RFC standards)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Illegal AgeI sites at 555 and 667 had to be removed considering the codon properties for e.coli. at bp 555: ccgg was changed to ccag. At bp 667: ccgg was changed to cggg.


Source

Discosoma striata (coral)

References